Product Overview

The Diazolidinyl Urea Glycine EDTA-K3 Blood Collection Tube is a specialized consumable designed for liquid biopsy and molecular diagnostics. In addition to the anticoagulant function of K3EDTA, the tube incorporates Diazolidinyl Urea and Glycine as stabilizing agents. This unique additive system effectively suppresses blood cell degradation after collection, reduces genomic DNA contamination, and preserves the integrity of circulating DNA (cfDNA and ctDNA) in plasma. It is particularly suitable for ctDNA testing, NGS sequencing, liquid biopsy, and tumor biomarker studies where high-quality samples are essential.

Key Advantages

• Dual Protection System: K3EDTA prevents coagulation; Diazolidinyl Urea and Glycine stabilize cells and reduce formaldehyde-related interference.
• cfDNA Stability: Effectively delays cell lysis at room temperature, minimizes genomic DNA contamination, and ensures reliable cfDNA extraction.
• Flexible Transport and Storage: Supports short-term room temperature storage and delayed centrifugation, allowing convenient long-distance shipping and centralized sample processing.
• Wide Compatibility: Compatible with mainstream molecular diagnostic platforms, NGS workflows, and nucleic acid extraction methods.
• Improved Reproducibility: Enhances test consistency and reduces variability caused by sample degradation.

The Use of Different Diazolidinyl Urea Glycine EDTA-K3 Blood Collection Tube

Working Principle

1. K3EDTA: Chelates calcium ions and prevents blood coagulation.
2. Diazolidinyl Urea: A slow-release stabilizer that reduces cell degradation and extends sample stability.
3. Glycine: Acts as a formaldehyde scavenger, minimizing side reactions and protecting nucleic acid integrity.

The combined action of these additives ensures that blood samples remain suitable for cfDNA analysis even without immediate centrifugation or refrigeration.

Technical Specifications

• Material: PET or glass tube body
• Dimensions: Common sizes include 13×75mm, 13×100mm, 16×100mm
• Draw Volume: 3ml / 4ml / 6ml / 10ml
• Additives: K3EDTA + Diazolidinyl Urea + Glycine
• Cap Color: Lavender (standard for EDTA tubes)
• Storage Condition: Room temperature transport; avoid extreme environments
• Packaging: 100 tubes/box, 1200 tubes/carton (customizable on request)

Application Fields

Thanks to its stabilizing system, the Diazolidinyl Urea Glycine EDTA-K3 Blood Collection Tube is widely used in clinical and research scenarios where high-quality nucleic acid samples are required:

• Liquid Biopsy
• Essential for cancer diagnosis and monitoring, this tube maintains cfDNA stability after collection and reduces genomic DNA contamination caused by cell lysis.
• Oncology Gene Testing and Molecular Diagnostics
• Ensures high sample integrity for mutation detection in genes such as EGFR, KRAS, and BRAF, improving sensitivity and reproducibility.
• Next-Generation Sequencing (NGS)
• Reduces genomic DNA contamination, providing more reliable sequencing results for WES, WGS, and targeted sequencing applications.
• cfDNA and ctDNA Monitoring
• Supports longitudinal sampling for therapy evaluation, relapse monitoring, and resistance mutation detection, ensuring consistent and comparable results.
• Non-Invasive Prenatal Testing (NIPT)
• Preserves the stability of fetal cfDNA by minimizing maternal cell degradation, improving the accuracy of prenatal genetic testing.
• Translational Research and Academic Studies
• Suitable for genomics, epigenetics, ctDNA research, and exosome studies, meeting the high requirements of scientific investigations.
• Routine Molecular Diagnostics
• Beyond oncology, the tube can also be applied in infectious disease testing, immunology, and genetic disorder screening, where the stability of cfDNA is important.

Instructions for Use

1. Gently invert the tube 8–10 times after collection to ensure complete mixing with additives.
2. Follow the IFU guidelines regarding timeframes for plasma separation.
3. Avoid exposing the tubes to extreme heat or freezing conditions.
4. The stabilizing system is intended for cfDNA applications only and should not be repurposed for other purposes.

Want to Learn More About the Diazolidinyl Urea Glycine EDTA-K3 Blood Collection Tube?

If you’re looking for a premium-quality, internationally compliant EDTA tube, we’re here to help. Contact us for samples, technical documentation, or OEM customization, or visit our official website. Let us support your clinical or laboratory needs with precision and reliability.